Renin (active) assay characteristics:
- Assay Principle: Sandwich ELISA
- Dynamic range: 0.8 – 128 pg/mL of active Renin
- Total assay time: 3.25 hours
- Sample volume: 50 µL EDTA Plasma or Serum
- Crossreactivity with Prorenin: 0,69 %
- Mean Intra Assay precision: 5.6 %
- Mean Inter Assay precision: 6.8 %
- Analytical sensitivity: 0.8 pg/mL
Clinical Revelance:
- Differential diagnosis of hyperaldosteronism
- Diagnosis of isolated deficit in mineral corticoids
- Detection of Renin-producing tumors (kidney and lung cancer)
- Diagnosis of malignant hypertonia (high blood pressure)
- Monitoring of glucocorticoid therapy
- Detection of physiological increase during pregnancy
Contact us today to find out more and speak to one of our experts about the benefits of DRG Renin ELISA
Renin (Active) ELISA – Comprehensive Guide
Introduction to Renin and Its Clinical Importance
Renin is a critical enzyme in the regulation of blood pressure and electrolyte balance. Understanding its role is essential for clinicians and researchers working in cardiovascular and renal health. Renin, secreted by the kidneys, initiates the renin-angiotensin-aldosterone system (RAAS), which controls blood pressure and fluid balance. Abnormal renin levels are associated with hypertension, heart failure, and kidney disorders.
Monitoring renin activity provides valuable insights into cardiovascular health and disease management, making it a key biomarker in clinical diagnostics.
What is Renin (Active) ELISA?
Renin (Active) ELISA is a highly sensitive immunoassay designed to quantify active renin levels in biological samples. This assay uses enzyme-linked immunosorbent technology to detect active renin with precision and reliability. It is widely used in clinical laboratories and research settings for accurate measurement of renin activity.
Renin ELISA kits offer a robust solution for detecting active renin, supporting diagnostic and research applications in hypertension and renal studies.
Renin is a protein (Mw of kDa) that belongs to the aspartic acid proteinase family of enzymes. Renin regulates the mean arterial blood pressure of the human body by cleavage of angiotensinogen into the precursor peptide angiotensin-1, which ultimately is processed by angiotension converting enzyme (ACE) to the active octapeptide angiotension-2.
Renin is produced constitutively as prorenin (inactive precursor with 386 amino acids) in the juxtaglomerular cells of the kidney. In response to the low blood pressure, low sodium chloride, or activity of the sympathetic nervous system, active Renin can be released either from a depot in the kidney or generated from prorenin. The blood concentration of prorenin is approx. 10-fold higher than active Renin.
Principle of the Renin ELISA Test
The principle behind the Renin ELISA test ensures specificity and sensitivity for active renin detection. The assay employs antibodies specific to active renin, immobilised on a microplate. Samples are added, and renin binds to these antibodies. A secondary enzyme-linked antibody facilitates colour development, which is measured spectrophotometrically.
The DRG active Renin ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.
The microtiter wells are coated with a monoclonal (mouse) antibody directed towards a unique antigenic site of the Renin molecule. An aliquot of patient sample containing endogenous active Renin is incubated in the coated well with assay buffer. After incubation the unbound conjugate is washed off and the wells are incubated with Enzyme Complex which is an anti-Renin antibody conjugated with horseradish peroxidase. The amount of bound peroxidase is proportional to the concentration of active Renin in the sample. Having added the substrate, the intensity of colour developed is proportional to the concentration of active Renin in the patient sample.
This method guarantees accurate quantification of active renin, making it ideal for clinical and research use.
Example of a Typical Standard Curve
| Standard | Optical Units (450 nm) |
| Standard 0 (0 pg/mL) | 0.09 |
| Standard 1 (4 pg/mL) | 0.19 |
| Standard 2 (16 pg/mL) | 0.44 |
| Standard 3 (32 pg/mL) | 0.78 |
| Standard 4 (64 pg/mL) | 1.14 |
| Standard 5 (128 pg/mL) | 2.48 |
Reproducibility
Intra Assay
The within assay variability is shown below:
| Sample | n | Mean (pg/mL) | CV (%) |
| 1 | 20 | 44.0 | 4.2 |
| 2 | 20 | 27.0 | 3.9 |
| 3 | 20 | 9.1 | 8.7 |
Inter Assay
The between assay variability is shown below:
| Sample | n | Mean (pg/mL) | CV (%) |
| 1 | 12 | 19.0 | 8.9 |
| 2 | 12 | 36.2 | 6.3 |
| 3 | 12 | 66.7 | 5.2 |
Applications in Clinical and Research Settings
Renin ELISA has diverse applications across medical and scientific fields. Clinically, it aids in diagnosing hypertension, adrenal disorders, and kidney diseases. In research, it supports studies on RAAS, cardiovascular health, and pharmacological interventions.
Its versatility makes Renin ELISA indispensable for both diagnostic and investigative purposes.
Kit Components and Specifications
The Renin ELISA kit includes all necessary reagents and materials for accurate testing. Typical components include pre-coated microplates, standards, controls, enzyme conjugates, substrate solutions, and wash buffers. Each kit is designed for ease of use and reproducibility.
Comprehensive kit components ensure reliable performance and user-friendly operation.
Performance Characteristics and Sensitivity
Renin ELISA kits are validated for high sensitivity and specificity. The assay demonstrates excellent linearity, low detection limits, and minimal cross-reactivity. Quality control measures ensure consistent performance across batches.
Superior sensitivity and reliability make this assay a trusted choice for renin measurement.