The DRG 25-OH Vitamin D (total) ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of total 25-OH Vitamin D (Vitamin D2 / D3) in serum or plasma.
25-OH-Vitamin D (total) assay characteristics:
- Assay Principle: Competitive ELISA
- Dynamic range: 3.22 – 120 ng/mL
- Total assay time: less than 2 h (60/30/15 minutes)
- Sample volume: 50 µL Serum or Plasma (EDTA, lithium heparin or citrate plasma)
- Mean Intra Assay precision: 3.7 %
- Mean Inter Assay precision: 7.13 %
- Specificity: 99.5% of 25-OH-Vitamin D3 and 97.4% of 25-OH-Vitamin D2
Clinical Benefit:
Vitamin D is a pro-hormone that has long been known for its important role in regulating bone mineralization. Moreover, in recent years, Vitamin D has become an assay of general health status, and there have been multiple publications linking Vitamin D deficiency to several disease states, such as cancer, cardiovascular disease, diabetes and autoimmune diseases.